To the guide region of the crRNA ( Brouns et al. The crRNA are then incorporated into effector complexes that identify and destroy invading nucleic acid that is complementary Transcripts from CRISPR loci are processed to generate short CRISPR RNAs (crRNA) ( Brouns et al. CRISPR loci are composed of repeat sequences separated by variable spacer sequences that are derived from foreign geneticĮlements ( Bolotin 2005 Mojica et al. Theseĭata suggest a mechanism for transcription-coupled DNA targeting by the Cmr complex and provide a unifying mechanism for allĬlustered regularly interspaced short palindromic repeats (CRISPRs) and their associated (Cas) proteins form the basis ofĪn inheritable and adaptable RNA-guided immune system in prokaryotes (for a recent review, see van der Oost et al. We also show that binding of a complementary ssRNA targetĪctivates an ssDNA-specific nuclease activity in the histidine–aspartate (HD) domain of the Cmr2 subunit of the complex. Thermotoga maritima can cleave an ssRNA target that is complementary to the CRISPR RNA. However, in vivo, it has been implicated in transcription-dependent DNA targeting. In the Type III-B system, the Cmr effector complex has been found to cleave ssRNA in vitro. This system represents an inheritable and adaptable immune system that is mediatedīy multisubunit effector complexes. Prokaryotes from invading genetic elements. The CRISPR (clustered regularly interspaced short palindromic repeat) system is an RNA-guided immune system that protects
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